Titlebook: Cardiac Tissue Engineering; Methods and Protocol Kareen L.K. Coulombe,Lauren D. Black III Book 2022Latest edition Springer Science+Business

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Methods for Transepicardial Cell Transplantation in a Swine Myocardial Infarction Model,ing lost muscle mass and contractile function in injured hearts. Early preclinical work with hPSC-CMs employed rodent models, but the field has recently advanced to transplantation studies in more translationally relevant large animal models including non-human primates and swine. The pig is a parti

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Defined Engineered Human Myocardium for Disease Modeling , Drug Screening, and Heart Repair, 3D engineered versus 2D monolayer and 3D aggregate cardiomyocyte cultures is a clearly advanced degree of maturation, which in many aspects resembles the postnatal rather than the embryonic or fetal heart, in the most advanced 3D culture formats. According to the desired in vitro (disease modeling

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Encapsulation of Pediatric Cardiac-Derived C-Kit + Cells in Cardiac Extracellular Matrix Hydrogel fts in recent studies and offer multiple benefits, such as easy isolation and autologous transplant. However, concerns about failure of engraftment and transient paracrine effects have thus far limited their use. To overcome these issues, an appropriate cell delivery vehicle such as a cardiac extrace

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,FRESH 3D Bioprinting a Ventricle-like Cardiac Construct Using Human?Stem Cell-Derived Cardiomyocytels are provided on FRESH support preparation, bioink preparation, dual-extruder needle alignment, print parameter selection, and post-processing. This protocol can also be adapted by altering the 3D model design, cell concentration, or cell type to FRESH 3D bioprint other cardiac tissue constructs.

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Quantifying Propagation Velocity from Engineered Cardiac Tissues with High-Speed Fluorescence Micro the same procedures can likely be used with human-induced pluripotent stem cell-derived cardiac myocytes, paving the way for patient-specific analysis of propagation due to features such as tissue architecture, substrate rigidity, genetic mutations, or drug treatments.

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,Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues, and calcium transients—to 3D heterotypic cardiac myocyte–fibroblast tissues allows for the generation and functional analysis of a large number of individual microtissues to provide greater throughput and high statistical power in analyses. Hundreds of microtissues in standard cell culture plates c