Titlebook: Cardiac Tissue Engineering; Methods and Protocol Milica Radisic,Lauren D. Black III Book 2014 Springer Science+Business Media New York 2014

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A Physical Space Powerfully Shaped by Man, tissue formation. The use of native extracellular matrix (ECM) is of special interest for the culture of cardiac stem and progenitor cells due to the presence of intrinsic regulatory factors regulating cardiac function. We describe here how to obtain native ECM hydrogels from porcine hearts for the

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https://doi.org/10.1057/9780333977866lture. Microenvironmental factors cue structural organization of the myocardium, which promotes the mechanical contractile properties and electrophysiological patterns seen in mature cardiomyocytes. Current nano- and microfabrication techniques, such as photolithography, generate simplified cell cul

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,Seamus Heaney: ‘the Appetites of Gravity’,issue engineering method requires cardiomyocytes, a liquid matrix that forms a gel, a casting mold, and a device that keeps the developing tissue in place. This protocol refers to neonatal rat heart cells as the cell source; the matrix of choice is fibrin, and the tissues are generated in rectangula

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https://doi.org/10.1057/9781137330390oach for induction of vascular networks embedded within cardiac tissue constructs. The construct is composed of human cardiomyocytes, endothelial cells (ECs), and embryonic fibroblast cells co-seeded onto highly porous three-dimensional (3D) scaffolds. The resulting vascularized cardiac constructs s

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Seamus Heaney: Neighbours and Strangers,sue regeneration. A temperature-responsive culture surface can be fabricated by grafting a temperature-responsive polymer, poly(.-isopropylacrylamide), onto a polystyrene cell culture surface. Cells cultured confluently on such a culture surface can be recovered as an intact cell sheet, and function

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Northern Irish Poetry and Domestic Spacetforms to study novel small molecules and cardiac disease at the tissue level. Yet, due to the immaturity of hPSC-CMs, there is a low fidelity between tissue-engineered cardiac tissues and adult cardiac tissues. To address this challenge, we have developed a platform that combines both physical and

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Northern Irish Poetry and Theologythe many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method.

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