Titlebook: Cardiac Tissue Engineering; Methods and Protocol Milica Radisic,Lauren D. Black III Book 2014 Springer Science+Business Media New York 2014

嫻熟

https://doi.org/10.1057/9781137271723to modulate cardiomyocyte properties. In this chapter we illustrate the use of the whole-cell patch clamp technique by recording hERG (human ether-à-go-go-related gene) currents and action potentials in ESC-derived cardiomyocytes and also examine the effects of hERG channel blocker, dofetilide.

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滔滔不絕的人

Isolation and Expansion of C-Kit-Positive Cardiac Progenitor Cells by Magnetic Cell Sorting,and clonogenic, can be harvested from mature tissue, and have the distinct advantages of autologous transplant and lack of tumor formation in a clinical setting. Here we focus on the isolation, expansion, and myocardial differentiation of rat CPCs. Brief adaptations of the protocol for isolation from mouse and human tissue are also provided.

消息靈通

Cell Tri-Culture for Cardiac Vascularization,howed microstructural details characteristic of cardiomyocytes and nascent vessels and exhibited synchronous beating activity in vitro. Upon implantation, stable grafts were formed presenting intense vascularization, with evidence of anastomosis between the pre-formed endothelial capillaries and host neovessels.

錯(cuò)

Patch-Clamp Technique in ESC-Derived Cardiomyocytes,to modulate cardiomyocyte properties. In this chapter we illustrate the use of the whole-cell patch clamp technique by recording hERG (human ether-à-go-go-related gene) currents and action potentials in ESC-derived cardiomyocytes and also examine the effects of hERG channel blocker, dofetilide.

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CIS

A Physical Space Powerfully Shaped by Man,ed to an alternating magnetic field of 40 Hz for 7 days. The magnetic stimulation per se promoted EC organization into capillary-like structures with no supplementation of angiogenic factors; in the non-stimulated constructs, the cells formed sheets or aggregates..This chapter describes in detail th

BULLY

https://doi.org/10.1057/9780333977866chniques for culturing stem cells and their cardiac derivatives on these substrates. Importantly, this wrinkled cell culture platform is compatible with both fluorescence and bright-field imaging; real-time physiological monitoring of CM action potential propagation and contraction properties can el

RALES

https://doi.org/10.1057/9780333977866diaphragm. Although the methods shown here are for injection of an acellular biomaterial, cells or other therapeutics could also be added to the injection for testing other regenerative medicine strategies.

GEON