Titlebook: Cardiac Gene Expression; Methods and Protocol Jun Zhang,Gregg Rokosh Book 2007 Humana Press 2007 DNA.In silico.Microarray.PCR.Polymerase Ch

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Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)r the cotransport of sodium predominantly with glutamine in exchange for hydrogen ions. The results suggested that a 20-fold increase in . expression occurs during ischemia, which reduced to baseline levels on reperfusion.

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Characterization of ,-Regulatory Elements and Transcription Factor Binding .-acting factor (antibody supershift assay), is used to identify proteins present in the DNA-protein complex. The gel-shift competition assay with an unlabeled probe containing a slightly different sequence is a powerful technique used to assess the sequence specificity and relative binding affinit

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Characterization of Cardiac Gene Promoter Activity that control gene expression under different physiological stresses have been further characterized in the context of heterologous promoters to demonstrate their “stand-alone” functional properties in the absence of confounding influences from other .-elements and their cognate transcription factor

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Book 2007eir cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome

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Inequalities for mixing processes,ribes the different technical procedures that are of crucial importance to carry on sensitive and specific . hybridization experiments in heart samples. The detection of transcripts within paraformaldehyde-fixed, paraffin-embedded cardiac tissue samples is illustrated here with the detection of cardiac sphingosine-1-phosphate receptor expression.

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Hybridizationribes the different technical procedures that are of crucial importance to carry on sensitive and specific . hybridization experiments in heart samples. The detection of transcripts within paraformaldehyde-fixed, paraffin-embedded cardiac tissue samples is illustrated here with the detection of cardiac sphingosine-1-phosphate receptor expression.