Titlebook: Capillary Electrophoresis: Instrumentation and Operation; Wim Kok Book 2000 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig/Wi

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1615-2255 the almost 20 years since its first appearance, Capil- Writing a monograph like this is a lot of work, even more lary Electrophoresis (CE) has become a standard analy- than I anticipated. Luckily, I could fall back on the tical separation technique in many laboratories. CE is theoretical and practic

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https://doi.org/10.1007/978-1-4614-7726-6tion of two adjacent peaks by their resolution (. .). Occasionally the similarity between chromatography and CE is overestimated, when an efficiency is expressed as the number of plates per meter. As will be shown below, plate numbers in CE do not increase proportionally with the capillary length.

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A. Tasidou,P. S. Efraimidis,V. Katose(s). In other respects there are cardinal differences between the two. Amperometric detection relies on the chemical characteristics of specific analytes, while with conductivity detection a bulk property of the separation medium is monitored. The first technique is therefore selective, the second inherently non-selective.

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Efficiency and Resolution,tion of two adjacent peaks by their resolution (. .). Occasionally the similarity between chromatography and CE is overestimated, when an efficiency is expressed as the number of plates per meter. As will be shown below, plate numbers in CE do not increase proportionally with the capillary length.

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Voltages and Currents,rical insulation of the various parts of the CE set-up becomes problematic, with spontaneous sparking and a risk of serious damage of sensitive electronic components as a result. A high precision and low drift of the applied voltage is of course important for the reproducibility of the migration times of analytes.

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