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Titlebook: Capillary Electrophoresis of Proteins and Peptides; Mark A. Strege,Avinash L. Lagu Book 2004 Humana Press 2004

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發(fā)表于 2025-3-21 19:30:04 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Capillary Electrophoresis of Proteins and Peptides
編輯Mark A. Strege,Avinash L. Lagu
視頻videohttp://file.papertrans.cn/222/221396/221396.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Capillary Electrophoresis of Proteins and Peptides;  Mark A. Strege,Avinash L. Lagu Book 2004 Humana Press 2004
描述Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established i
出版日期Book 2004
版次1
doihttps://doi.org/10.1385/159259798X
isbn_softcover978-1-61737-307-7
isbn_ebook978-1-59259-798-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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發(fā)表于 2025-3-21 23:17:27 | 只看該作者
https://doi.org/10.1007/978-1-4614-0779-9he role of interactions between charged groups on protein stability and ligand binding are also given. The power of this approach lies in the ability to isolate protein charge as an independent and measurable variable in the study of protein stability and function.
板凳
發(fā)表于 2025-3-22 01:52:12 | 只看該作者
Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions,nd injection ACE (MSLIACE) are used to probe the binding of ristocetin A (Rist A) and vancomycin (Van) from . to .-Ala-.-Ala terminus peptides and carbonic anhydrase B (CAB, E.C.4.2.1.1) to arylsulfonamides.
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Vandana Kumari Jha,Soubhik Kumar Bhaumik those utilizing Si-O and Si-C covalent bonds. The apparatus and methods are presented for capillary coating using Si-O and Si-C chemistry. Furthermore, procedures are described for monitoring the quality of coating.
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發(fā)表于 2025-3-22 20:19:50 | 只看該作者
Robert Castelo,Alberto Roveratohe typical ICHQ2A parameters, including accuracy, linearity, LOQ, precision, robustness/ruggedness, and specificity. All validation parameters met the acceptance criteria defined in the validation protocol.
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發(fā)表于 2025-3-23 00:10:28 | 只看該作者
A. A. Ojo,W. M. Cranton,I. M. Dharmadasaolumn-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.
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Capillary Electrophoresis of Proteins in a Quality Control Environment,he typical ICHQ2A parameters, including accuracy, linearity, LOQ, precision, robustness/ruggedness, and specificity. All validation parameters met the acceptance criteria defined in the validation protocol.
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