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Titlebook: Capillary Electrophoresis of Proteins and Peptides; Methods and Protocol Nguyet Thuy Tran,Myriam Taverna Book 2016 Springer Science+Busines

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書(shū)目名稱(chēng)Capillary Electrophoresis of Proteins and Peptides
副標(biāo)題Methods and Protocol
編輯Nguyet Thuy Tran,Myriam Taverna
視頻videohttp://file.papertrans.cn/222/221395/221395.mp4
概述Includes cutting-edge methods and protocols for the study of CE of proteins and peptides.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Capillary Electrophoresis of Proteins and Peptides; Methods and Protocol Nguyet Thuy Tran,Myriam Taverna Book 2016 Springer Science+Busines
描述This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and exhaustive, .Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols. serves both beginners and experts with a collection of the current and most active topics in this vital field of s
出版日期Book 2016
關(guān)鍵詞CE-MS; Intact proteins; Detection methods; Therapeutic proteins; Quality control; Vaccine proteins; Glycop
版次1
doihttps://doi.org/10.1007/978-1-4939-4014-1
isbn_softcover978-1-4939-8153-3
isbn_ebook978-1-4939-4014-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Amy Defnet,Ramon Martinez III,Paul ShapiroHere we describe a highly reproducible CZE method, relying on a cationic coating allowing separation and identification of a complex mixture of four compounded mAbs widely used in cancer therapy (cetuximab, rituximab, bevacizumab, and trastuzumab).
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https://doi.org/10.5822/978-1-61091-202-0ectrometry (ESI-MS), it is a powerful tool offering the opportunity of high selectivity and sensitivity combined with the possibility to identify and characterize intact proteins. In this protocol, we demonstrate a screening method for intact proteins based on capillary zone electrophoresis (CZE) se
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https://doi.org/10.5822/978-1-61091-202-0c point. CIEF coupled online with MS is regarded as a promising alternative to 2-D PAGE for fast proteome analysis with high-resolving capabilities and enhanced structural information without the drawbacks of conventional slab-gel electrophoresis. However, online coupling has been rarely described,
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