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Titlebook: Capillary Electrophoresis Guidebook; Principles, Operatio Kevin D. Altria Book 1996 Humana Press 1996

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書目名稱Capillary Electrophoresis Guidebook
副標(biāo)題Principles, Operatio
編輯Kevin D. Altria
視頻videohttp://file.papertrans.cn/222/221389/221389.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Capillary Electrophoresis Guidebook; Principles, Operatio Kevin D. Altria Book 1996 Humana Press 1996
描述This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap- idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro- vided for individual analyses. The intention is to provide general guide- lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth- ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.
出版日期Book 1996
版次1
doihttps://doi.org/10.1385/0896033155
isbn_softcover978-1-61737-011-3
isbn_ebook978-1-59259-539-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1996
The information of publication is updating

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Quantitation Procedures a plot of UV absorbance vs time. Therefore, HPLC data handling and peak integration packages are generally applicable to CE. Main component assay may be performed using external and internal standardization or by standard addition. Impurity data can be calculated and reported as either % w/w or % a
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Method ValidationThe extent of tests applied will vary according to the nature of the application and the extent of intended usage. Generally, such aspects as accuracy, precision, reproducibility, linearity, robustness, and method of transfer are evaluated during validation.
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