Titlebook: Candida Species; Methods and Protocol Richard Calderone Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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Plasmid-Based CRISPR–Cas9 Editing in Multiple , Speciescombination with a repair template for disrupting gene function by insertion of a premature stop codon or by gene deletion. The plasmids are easily lost in the absence of selection, allowing scarless gene editing and minimizing detrimental effects of prolonged Cas9 expression.

尾隨

A Simple Method for Growth of , Biofilms Under Continuous Media Flow and for Recovery of Biofilm Disories. Biofilms developed using this system are robust and particularly suitable for studies requiring large amounts of biofilm (dispersed) cells for downstream analyses. Importantly, this apparatus mimics in vivo flow conditions, thereby making it a physiologically relevant model.

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The Techniques Innovated to Support NSE,pper and how to browse large-scale datasets using the JBrowse genome browser. Finally, it shows how to search and retrieve data for user-defined sets of genes using the Advanced Search and Batch Download tools.

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https://doi.org/10.1007/978-1-4419-7326-9e ever-increasing number of applications to the study of pathogenesis, drug resistance, gene expression, and host pathogen interaction and drug discovery. Here, we describe the use of plasmid-based systems for high efficiency CRISPR-Cas9 gene editing into any strain of four non-. species, namely, .,

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