Titlebook: Cancer Vaccines; Methods and Protocol Michael J.P. Lawman,Patricia D. Lawman Book 2014 Springer Science+Business Media New York 2014 Cancer

ferment

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Fast Monocyte-Derived Dendritic Cell-Based Immunotherapyn vitro culture (fast-DC). Mature fast-DC are as effective as mature standard-DC (generated in 7–10 days of in vitro culture) in priming and propagation of antigen-specific T-cell responses. The use of fast-DC not only reduces labor and supply cost, as well as workload and time, but also increases t

Expediency

Intratumoral Injection of BCG-CWS-Pretreated Dendritic Cells Following Tumor Cryoablation induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wa

CURL

Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccinesr cells by pro-inflammatory cytokines. Evidence is accumulating that the CD1d-iNKT cell axis can be effectively used to potentiate DC-based cancer vaccines. Here, we provide a detailed methodology for the generation of (CD1d-expressing) monocyte-derived DC (moDC) and their subsequent loading with th

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Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptmunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR. T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8. and CD4. T

Mast-Cell

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1064-3745 ail essential for reproducible results.Contains key notes an.Cancer Vaccines: Methods and Protocols. explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms,

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https://doi.org/10.1007/978-981-10-3872-3 of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC generated ex vivo from patients with gastrointestinal cancer.

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https://doi.org/10.1007/978-981-10-3872-3r designing an effective tumor vaccine protocol. Here, we describe the stimulation of purified splenic- or bone marrow-derived DC with recombinant interleukin-15 (IL-15) in the presence of intact soluble antigen from metastatic lymphoma tumor cells in an experimental animal model.