Titlebook: Cancer Nanotechnology; Methods and Protocol Stephen R. Grobmyer,Brij M. Moudgil Book 2010 Humana Press 2010 Gold nanorods, nanocages and na

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Polymeric Nanoparticles for Drug Delivery,d delivery technologies are becoming increasingly important as a scientific area of investigation. In cancer, targeted polymeric NPs can be used to deliver chemotherapies to tumor cells with greater efficacy and reduced cytotoxicity on peripheral healthy tissues. In this chapter, we describe the met

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Preparation and Characterization of Doxorubicin Liposomes,s), containing polyethylene glycol-conjugated lipid, which can form a hydro-layer around liposomes bilayer, have a long circulation time and hence result in enhanced drug efficiency. Doxorubicin (DOX), an effective anticancer drug, can be loaded into liposomes by transmembrane pH gradient method to

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PEGylated Nanocarriers for Systemic Delivery,livery. PEGylation simply refers to the modification of particle surface by covalently grafting, entrapping, or adsorbing PEG chains of vivid length. However, limitation of simple adsorption being easy displacement of the coating layer in vivo, covalent mode for PEGylation of nanoparticles is mostly

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,Nanoparticle–Aptamer Conjugates for Cancer Cell Targeting and Detection, iterative selection protocol called SELEX. Several small molecules and proteins have been used as targets. Recently, a variant of this methodology, known as cell-SELEX, has been developed for a new generation of aptamers, which are capable of recognizing whole living cells. We have used this method

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Magnetic Aerosol Targeting of Nanoparticles to Cancer: Nanomagnetosols,n the lungs, it may be advantageous to control aerosol deposition and target aerosols to diseased or disease-causing lung tissue and cellular structures in order to maximize drug potency and minimize side effects in unaffected tissue. We have recently investigated a novel method which brings aerosol

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LHRH-Targeted Nanoparticles for Cancer Therapeutics,r dendrimer as well as PAMAM–paclitaxel conjugate are described. The advantages of developed nanocarriers include but are not limited to (1) internal cationic charges for the complexation with small interfering RNA or antisense oligonucleotides and their protection from the degradation in systemic c

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Book 2010s a volume in the highly successful .Methods in Molecular Biology.? series, the protocols chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding