Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Narendra Wajapeyee Book 2014Latest edition Springer Science+Business Media New York 20

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,Interrogation of In Vivo Protein–Protein Interactions Using Transgenic Mouse Models and Stable Isot genes and proteins. Here we discuss the methods we have recently employed to characterize protein–protein interactions and posttranslational modifications in tagged knock-in mouse models. These methods can be broadly applied to other systems for various applications in both basic and translational science.

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https://doi.org/10.1007/978-981-97-0109-4or the acquisition of comprehensive information of the methylation landscape in diseases like cancer. Data generated by this approach is typically reproducible and often covers between 65 and 75 % of the whole genome.

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https://doi.org/10.1007/978-981-97-0109-4m total RNA samples and on-site biochemical reactions, coupled with bead-based detection in 96-well format for hundreds of miRNAs per sample. With low-cost, high-throughput, high detection specificity, and flexibility to profile both small and large numbers of samples, this protocol can be adapted in a wide range of laboratory settings.

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