Titlebook: Calpain; Methods and Protocol Jeannette S. Messer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 proteases.en

foreign

哄騙

Identification of Calpain-Activated Protein Functionsbstrates. The cleavage of some substrates can produce active fragments that perform functions that are different from those performed by the full-length proteins. Therefore, cleavage by calpains can operate as a posttranslational modification and increase the functional diversity of target proteins.

finale

音樂學者

Isolation of Endogenous Calpastatinhibitory activity against calpain in the presence of Ca. to specifically identify the protein. Thus, the purification steps necessary to obtain the inhibitor protein were originally designed to obtain calpain from the same tissue. For this reason, in addition to calpastatin purification, we also inc

易于

Immunoblotting for Calpastatin Expression calpastatin isoforms can vary by tissue, can be modified by partial digestion, and can undergo posttranslational modifications. Here we describe a method for more precise evaluation of calpastatin expression by combining immunoblot analysis with an assay for the inhibitory activity of a single calp

手銬

束以馬具

Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin Using Anion-Exchange Chromatographs that effectively bind with the DEAE Sephacel and separate well using a stepwise, increasing gradient of NaCl to elute the proteins. Calpastatin binds most weakly with the column matrix, so it elutes first, followed by calpain-1 and, finally, calpain-2.

Adenoma

Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activityvoid of dye when stained with Coomassie Brilliant Blue G-250. We describe here how calpains can be extracted from muscle tissue and assayed for activity using this technique. This technique is also generally applicable to lysates from other types of cells or tissues.

抱狗不敢前

Mara B. Antonoff,Ashok K. Salujas that effectively bind with the DEAE Sephacel and separate well using a stepwise, increasing gradient of NaCl to elute the proteins. Calpastatin binds most weakly with the column matrix, so it elutes first, followed by calpain-1 and, finally, calpain-2.

Gerontology