Titlebook: Calcium Signaling Protocols; David G. Lambert Book 2005Latest edition Humana Press 2005 ATP.ATPase.Calcium.Endoplasmatisches Reticulum.Nuc

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Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assayroduce the second messengers, inositol(1,4,5)trisphosphate [Ins(1,4,5)P.] and diacylglycerol (.). Ins(1,4,5)P. interacts with specific receptor populations of ligand-gated channels to mobilize nonmitochondrial intracellular calcium (Ca.) stores (.). Because Ins(1,4,5)P. is very hydrophilic, it canno

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Microinjection of ,-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adhosphoinositidase Cs (.). These enzymes catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to produce the second messengers, .-inositol(1,4,5)trisphosphate [Ins(1,4,5)P.] and diacylglycerol (.). Ins(1,4,5)P. interacts with a specific receptor that is a ligand-gated channel that allows m

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https://doi.org/10.1385/1592599494ATP; ATPase; Calcium; Endoplasmatisches Reticulum; Nucleotide; molecular biology; protein; reproducible tec

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Calcium Signaling Protocols978-1-59259-949-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Video-Boom Neue Medien im Vormarsch,An elevation in intracellular calcium concentration ([Ca.].) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (.,.). The pattern of elevation in [Ca.]. and response to that elevation is dependent on the agonist and the cell type.

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Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2An elevation in intracellular calcium concentration ([Ca.].) acts to trigger a range of cellular events including neurotransmitter release, muscle contraction, and oocyte fertilization (.,.). The pattern of elevation in [Ca.]. and response to that elevation is dependent on the agonist and the cell type.

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Bernd Schorb,Hans-J?rg Stiehlermulation of receptors, belonging to almost all the classes so far described, will result in changes in [Ca.].. These changes might be directly induced by either Ca.-influx or Ca.-mobilization from intracellular stores, or indirectly by a number of other mechanisms (.–.). The development of fluoresce

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