Titlebook: CRISPR-Cas Methods; Volume 2 M. Tofazzal Islam,Kutubuddin Ali Molla Book 2021 The Editor(s) (if applicable) and The Author(s), under exclus

減震

Jürgen S?keland,Angelika S?kelandbinase polymerase amplification, target gene detection with Cas12a, and lateral flow assay. Owing to its simplicity, efficiency, robustness, and low-cost, this all-paper-based Cas12a DNA test method could be easily applied in field for crop disease diagnosis and GMO test.

艱苦地移動

民間傳說

GREEN

In Silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing, structure in target recognition and efficacy of CRISPR-Cas systems, it is vital to assess the gRNA secondary structure. Here, we describe a protocol to determine the gRNA secondary structure using RNA-fold software and explain how to interpret the results.

chandel

PAD416

Targeted Base Editing in Soybean Using a CRISPR-Cas9 Cytidine Deaminase Fusion,posed of nCas9, a rat cytidine deaminase enzyme APOBEC1, and a uracil DNA glycosylase inhibitor (UGI). The CBE was constructed in the PTF101 vector background. The PTF101-CBE has successfully achieved single-base alteration in soybean. Here, we present a detailed protocol of this base editor to generate a point mutation in soybean.

fidelity

Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato,ovide a detailed protocol for highly efficient plant transformation and regeneration in tomato to generate stable lines along with variant analysis of putative mutant lines followed by phenotypic analysis for the trait-of-interest. From the design of target site, generation of genome-edited tomato plants can be realized in 16–20?weeks.

香料

CRISPR-Cas12a-Based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants,binase polymerase amplification, target gene detection with Cas12a, and lateral flow assay. Owing to its simplicity, efficiency, robustness, and low-cost, this all-paper-based Cas12a DNA test method could be easily applied in field for crop disease diagnosis and GMO test.

Criteria

啜泣