Titlebook: CRISPR-Cas Methods; M. Tofazzal Islam,Pankaj K. Bhowmik,Kutubuddin A. Book 2020 Springer Science+Business Media, LLC, part of Springer Na

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書目名稱CRISPR-Cas Methods影響因子(影響力)




書目名稱CRISPR-Cas Methods影響因子(影響力)學(xué)科排名




書目名稱CRISPR-Cas Methods網(wǎng)絡(luò)公開(kāi)度




書目名稱CRISPR-Cas Methods網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書目名稱CRISPR-Cas Methods被引頻次




書目名稱CRISPR-Cas Methods被引頻次學(xué)科排名




書目名稱CRISPR-Cas Methods年度引用




書目名稱CRISPR-Cas Methods年度引用學(xué)科排名




書目名稱CRISPR-Cas Methods讀者反饋




書目名稱CRISPR-Cas Methods讀者反饋學(xué)科排名

熱情贊揚(yáng)

Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific ExpressioNA double-strand breaks. However, up to now its application was mostly restricted to nonhomologous end-joining-mediated targeted mutagenesis. In contrast, precise genome modifications using a suitable donor sequence for homologous still pose a particular challenge in plants, as NHEJ is the dominant

aesthetic

Rice Gene Knockout or Downregulation through CRISPR-Cas9, a powerful tool for improving abiotic and biotic stress tolerance and increasing yield in rice. Valuable agronomical traits and functional study of a novel gene can be obtained by targeting single or multiple mutations in the rice genome. Here we describe the sgRNA design, cloning, and assembly of

concentrate

拱形大橋

CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species ,explored in the model grass species . Selection of a single-guide RNA based on location in the gene and low risk of potential off-target effects is initially presented, with the goal of generating INDELS in a single-gene target. Next, the use of the polycistronic t-RNA/gRNA system to simultaneously

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An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (,re created mainly by random mutagenesis, which produced many undesirable mutations and genomic rearrangements. With the emergence of programmable site-specific nucleases, mutations can be introduced precisely at specific genomic locations. CRISPR-Cas9 technology represents the most versatile and eff

語(yǔ)源學(xué)

octogenarian

Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detectioditing. The recent efforts of scientists allow to improve current genome engineering techniques, which are used to generate modified cell lines for functional studies. Here we describe, in detail, the protocol for DNA-free CRISPR-Cas9-mediated gene editing in a human liver cell line. Briefly, we use