Titlebook: CRISPR Guide RNA Design; Methods and Protocol Tudor A. Fulga,David J. H. F. Knapp,Quentin R. V. Book 2021 Springer Science+Business Media,

只看該作者 · 發(fā)表于 2025-3-27 19:16:08

Harnessing tRNA for Processing Ability and Promoter Activityengineered tRNAs can be used to process gRNAs from Pol-II transcripts, thus enabling spatial/temporal control of gRNA expression. Here we describe the design, cloning, and testing of tRNA scaffolds for both Pol-III-driven expression of different levels of gRNAs, and for processing gRNAs from Pol-II transcripts.

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只看該作者 · 發(fā)表于 2025-3-28 05:10:49

https://doi.org/10.1007/978-3-662-25288-8the presence of a user defined inducer. In this chapter, I cover the know-how relating to the design and synthesis of iSBH-sgRNAs, as well as the implementation in mammalian cells of inducible CRISPR-TR strategies based on this technology.

只看該作者 · 發(fā)表于 2025-3-28 08:53:19

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