Titlebook: C. elegans; Methods and Applicat Kevin Strange Book 2006 Humana Press 2006 SNP.microscopy.molecular biology.mutant.transcription

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J. Meril?,B. C. Sheldon,L. E. B. Kruuk. Quantitative parameters of . that can be assayed include growth, size, progeny production, behavior, and mortality. Qualitative parameters of toxicity, such as changes in appearance or movement, can also be determined. This chapter describes four assays we have used for analyzing the toxic effects

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Matthias Adler,Sophie Waldschmidtgenetically encoded markers like the fluorescent proteins of the GFP family became the reporter of choice for gene expression studies and protein localization. The small size and transparency of the worm allows the visualization of in vivo dynamics, which increases the number of potential applicatio

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Prototype Applications of Gas Flowsl is that . is amenable to genetic manipulation. The complete genome sequence, advances in mass spectrometry, and RNA-mediated interference (RNAi) are now providing a technical infrastructure that complements biochemical and proteomic approaches. This chapter describes simple methods for sperm and o

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Multiscale Modeling of Gas Flows, an adult . hermaphrodite approaches its widest girth of approx 0.1 mm. In theory, an entire living adult animal can be physically immobilized instantly in amorphous ice by HPF, thus, providing a unique opportunity to examine cellular architecture with exquisite spatial preservation. The following

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Numerical Methods for Continuum Simulationhes allow for precise quantitative definitions of behavioral phenotypes that have traditionally been subjectively described by individual observers. Automated systems also facilitate the analysis of behaviors that occur over long time scales or are difficult to detect by eye. Here we describe the de

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