Titlebook: Brown Adipose Tissue; Methods and Protocol David A. Guertin,Christian Wolfrum Book 2022 The Editor(s) (if applicable) and The Author(s), un

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https://doi.org/10.1007/978-3-662-64772-1 as Adipo-Clear, highlighting key steps in the protocol as well as important technical considerations. This versatile approach can provide entirely new insights into adipose tissue biology in health and disease.

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https://doi.org/10.1007/978-1-4039-1921-2ative data better reflects physiologically important alterations. We stress the importance of performing experiments at thermoneutrality in order to obtain clinically relevant data and stress that true thermogenic agents may be overlooked if this is not done.

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https://doi.org/10.1007/978-981-13-7848-5escribed here include norepinephrine-induced thermogenic gene expression using qPCR; norepinephrine-induced mitochondrial uncoupling using the Seahorse XFe96 Analyzer, and norepinephrine-induced expression of . using the RNAscope. Technology.

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Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes,. This method enables metabolic roadmaps to be drawn and pathway fluxes to be inferred for each nutrient tracer within BAT and its application could uncover new metabolic pathways not previously appreciated for BAT physiology.

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https://doi.org/10.1007/978-1-349-89053-8energic receptor (AR) agonist, mirabegron. Acute pharmacological stimulation has enabled quantification of whole-body BAT volume and metabolic activity using PET/CT imaging, and chronic stimulation increases these properties of BAT over time.