Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na

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書目名稱Brain Development影響因子(影響力)




書目名稱Brain Development影響因子(影響力)學(xué)科排名




書目名稱Brain Development網(wǎng)絡(luò)公開度




書目名稱Brain Development網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Brain Development被引頻次




書目名稱Brain Development被引頻次學(xué)科排名




書目名稱Brain Development年度引用




書目名稱Brain Development年度引用學(xué)科排名




書目名稱Brain Development讀者反饋




書目名稱Brain Development讀者反饋學(xué)科排名

galley

廚師

Computational Methods in Applied Sciencesthe fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene?knockout.

FUSC

毛細(xì)血管

CRISPR/Cas9 Genome Editing to Study Nervous System Development in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to replace the endogenous genome sequence with a designed template of interest. We here provide a protocol to generate an FRT/FLP-based conditional GFP or HA-flagged gene?knockout.

indoctrinate

A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hyeps of cell fate specification to be followed at a single cell resolution. This protocol describes preparation of . embryos, classical in situ hybridization protocol coupled with nuclear staining, and a guide to identify gene expression in specific precursors of the developing brain at neural plate stages of development.

態(tài)度暖昧

多樣

凝視

Vladimir Leble,George N. Barakosrotocols for wholemount and flat preparations of . embryos, which allow a more detailed analysis of gene expression patterns in relation to the cellular context of the early brain (and facilitate the identification of individual brain neuroblasts) using conventional light microscopy.

MEET

Zhengshun Cheng,Torgeir Moan,Zhen Gaoin multiple colors. This is followed by a detailed protocol as to how to prepare samples for imaging. Finally, we provide specifications to facilitate multichannel image acquisition using confocal microscopy.