Titlebook: Bone Research Protocols; Aymen I.‘Idris Book 20193rd edition Springer Science+Business Media, LLC, part of Springer Nature 2019 osteoclast

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2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studiesell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

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Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridizationption and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA?) probes.

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The Ex Vivo Organ Culture of Boneight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skele

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Ex-Vivo Model Systems of Cancer-Bone Cell Interactionsic and dynamic” culture conditions and their potential use to understand cellular and molecular mechanisms as well as drug testing and discovery. An additional focus of this chapter is to provide details of how to incorporate varying oxygen tension, viz., hypoxic, normoxic, and hyperoxic, in such st