Titlebook: Bone Marrow Environment; Methods and Protocol Marion Espéli,Karl Balabanian Book 2021 Springer Science+Business Media, LLC, part of Springe

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Imaging of Bone Marrow Plasma Cells and of Their Nichesheir interaction with other cell types in situ. We achieved to successfully establish longitudinal imaging of the bone marrow (LIMB) that is based on an implantable endoscopic device. In this chapter, basic approaches on how to investigate plasma cell–stroma interaction and surgical implantation procedures are introduced.

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Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cellsscovered in the bone marrow and studied for their capacity to maintain hematopoietic cells. We will describe here methods to isolate, culture, and bank MSC from human bone marrow. Then, characterization protocols by flow cytometry, clonogenic assays and doubling time evaluation will be developed. Fi

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Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cellslecular components required for their lifelong maintenance and differentiation. Although HSCs have been extensively analyzed and characterized, their ex vivo expansion, which constitutes a promising approach for therapeutic development in regenerative medicine, remains challenging. Here, we describe

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Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cellsze subsets of hematopoietic stem and progenitor cells isolated from long bones of mice. We further show that this method allows for comparing JAM-C protein expression between subsets of hematopoietic stem and progenitor cells.