Titlebook: Bone Marrow Environment; Methods and Protocol Marion Espéli,Karl Balabanian Book 2021 Springer Science+Business Media, LLC, part of Springe

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https://doi.org/10.1007/978-94-010-2770-0ation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.

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Willie van Peer,Aikaterini Nousit bone marrow components. We provide a guide for conducting adoptive transfer experiments of fluorescently labeled leukocytes and visualizing their location in the bone marrow with respect to the bone marrow vasculature. This method presents a quick, easy, and inexpensive approach to image the bone marrow in three dimensions.

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Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cellsnally, in vitro MSC culture and differentiation into osteoblasts, adipocytes, and chondrocytes will be explained. Thus, this chapter will detail all bases to work on MSC with consensus and clear methods and protocols.

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Culture, Expansion and Differentiation of Mouse Bone-Derived Mesenchymal Stromal Cellss. Here we describe the minimum requirements and an efficient method for isolation, expansion of mouse bone-derived multipotent mesenchymal stromal cells and their differentiation into osteoblasts, responsible for the bone matrix synthesis and mineralization.

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Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrowt bone marrow components. We provide a guide for conducting adoptive transfer experiments of fluorescently labeled leukocytes and visualizing their location in the bone marrow with respect to the bone marrow vasculature. This method presents a quick, easy, and inexpensive approach to image the bone marrow in three dimensions.

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https://doi.org/10.1007/978-94-010-2770-0ate BMSCs for flow cytometry analyses and subsequent FACS. Use of transgenic reporter lines facilitates FACS-based isolation of BMSCs, aiding to uncover fundamental characteristics of these diverse cell populations.

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Introduction: the Mirror and the Image,treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow trephine biopsies, improving the antigen-antibody recognition and preserving the morphology and the architecture of the bone marrow microenvironment.