Titlebook: Biotechnology and Environmental Science; Molecular Approaches S. Mongkolsuk,P. S. Lovett,J. E. Trempy Book 1992 Springer Science+Business M

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Host-Vector System and Reverse Genetics in A Non-Conventional Yeast,es not have plasmids, the isolation of an ARS site from the genome of C maltosa was attempted so as to construct vectors. Several host-vector systems were constructed to promote reverse genetics in this non-conventional yeast as described in our recent papers shown below.

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Role of the αA-Crybp1 Site in Lens-Specific Expression of the αA-Crystallin Genessed in a lens-specific manner, while the αB gene is expressed in numerous tissues (see 24 for review). A combination of transgenic mouse (28), mutagenesis, and cDNA cloning experiments (20) have implicated the αA-CRYBP1 site (5′- GGGAAATCCC-3′ at-66 to-57) in the lens-specific expression of the mouse α-crystallin gene.

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Modification of Specific Chromosomal Loci in Mammalian Cells by Recombination, or a gene) are examined in great detail through a series of narrowly defined questions and experimental protocols. This approach can illuminate many phenotypic characteristics of complex systems, including whole organisms, if those phenotypes are the direct consequence of a single gene’s function.

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Role of the αA-Crybp1 Site in Lens-Specific Expression of the αA-Crystallin Geneeview). The two a-crystallins (αA and (αB) are present in all vertebrates, and arose from duplication of a common ancestral gene. The αA gene is expressed in a lens-specific manner, while the αB gene is expressed in numerous tissues (see 24 for review). A combination of transgenic mouse (28), mutage

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