Titlebook: Biopolymer Methods in Tissue Engineering; Anthony P. Hollander,Paul V. Hatton Book 2004 Humana Press 2004

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Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound He, fragments D and E) were shown to be chemotactic to macrophages, human fibroblasts, and endothelial cells (.–.). Thrombin has also been shown to exert proliferative and adhesive effects on cultured cells (.–.). We previously demonstrated that covalently coating inert Sepharose beads with either fib

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Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering,-glucuronic acid and N-acetylglucosamine. It is the only GAG that lacks an associated protein moiety and sulfate groups. HA is a highly conserved and widely distributed polysaccharide. In a variety of mammalian tissues, it exerts structural functions because of its peculiar physicochemical propertie

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Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2,nd regeneration of skeletal tissues. In particular, recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to induce new bone formation by differentiation of mesenchymal progenitor cells into osteoblasts (.). BMPs act locally, and thus for a clinically beneficial outcome, a carrier

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Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tisssue or an organ. Engineered tissues are produced by using cells that are manipulated through their extracellular environment to develop living biological substitutes for tissues that are lacking or malfunctioning (.–.). Many different strategies may be used to accomplish this goal. Among the most im

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Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Enginetissues. Biological tissues consist of cells situated within a complex molecular framework known as the extracellular matrix (ECM) with an integrated vascular system for oxygen or nutrient supply. In soft tissues, native ECMs consist mainly of collagens, proteoglycans, glycosaminoglycans (GAGs), lam

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Modification of Materials With Bioactive Peptides,rowth factors. Such materials can be designed to induce specific biological responses desired in applications such as tissue engineering and regenerative medicine. For example, transforming growth factor-beta (TGF-β) has been immobilized to polymeric scaffold materials to increase the synthesis of e

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Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue scaffold prior to implantation. The bioresorbable scaffold must be biocompatible and porous in order to facilitate rapid vascularization and growth of newly formed tissue (.–.). During the in vitro culture period, the seeded cells proliferate and secrete tissue-specific extracellular matrix (ECM).