Titlebook: Biophysics of Membrane Proteins; Methods and Protocol Vincent L. G. Postis,Adrian Goldman Book 2020 Springer Science+Business Media, LLC, p

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The Making of the Twentieth Centurying the mechanisms behind protein function. Herein we describe a fluorescence-based method that enables the measurement of distances between specific domains within a protein and how it might change during protein function. The method is transition metal ion F?rster resonance energy transfer (tmFRET

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Vincent L. G. Postis,Adrian GoldmanIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/188370.jpg

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Quantifying the Interaction of Phosphite with ABC Transporters : MicroScale Thermophoresis and a Novf the binding affinity between proteins and ligands. To demonstrate its applicability for periplasmic proteins, we provide a detailed protocol for determination of the binding affinity of phosphite to three ABC transporter periplasmic-binding proteins from environmental microorganisms. ABC transport

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Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomesbic environment and allows?one to tackle their study from both functional and structural points of view. Methods for their rapid and efficient reconstitution have been known for a?long?time but the quality and dispersity of the resulting suspensions is often overlooked. Here we describe our routine

MEAN

Functional Characterization of SLC Transporters Using Solid Supported Membranes protocol to the Na.-coupled sugar transporter SGLT1, the organic cation transporter OCT2, the Na./Ca. exchanger NCX, and the neuronal glutamate transporter EAAT3..The assay was developed for the commercially available SURFE.R N1 instrument (Nanion Technologies GmbH) which applies solid supported me

BRIDE

Thermostability Assays: a Generic and Versatile Tool for Studying the Functional and Structural Propa thiol-reactive fluorochrome N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM) can be used. An unfolding profile is obtained when the fluorochrome becomes fluorescent on reaction with cysteine residues that have been exposed during thermal denaturation of the protein population. The