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Titlebook: Biophysics and the Challenges of Emerging Threats; Joseph D. Puglisi Conference proceedings 2009 Springer Science+Business Media B.V. 2009

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期刊全稱Biophysics and the Challenges of Emerging Threats
影響因子2023Joseph D. Puglisi
視頻videohttp://file.papertrans.cn/189/188363/188363.mp4
發(fā)行地址Presents state of the art information on NMR spectroscopy, and its place in the broader field of biophysics.No other monograph presents such a wide range of topics, including NMR spectroscopy, protein
學科分類NATO Science for Peace and Security Series B: Physics and Biophysics
圖書封面Titlebook: Biophysics and the Challenges of Emerging Threats;  Joseph D. Puglisi Conference proceedings 2009 Springer Science+Business Media B.V. 2009
影響因子Single-molecule techniques eliminate ensemble averaging, thus revealing transient or rare species in heterogeneous systems [1–3]. These approaches have been employed to probe myriad biological phenomena, including protein and RNA folding [4–6], enzyme kinetics [7, 8], and even protein biosynthesis [1, 9, 10]. In particular, immobilization-based fluorescence te- niques such as total internal reflection fluorescence microscopy (TIRF-M) have recently allowed for the observation of multiple events on the millis- onds to seconds timescale [11–13]. Single-molecule fluorescence methods are challenged by the instability of single fluorophores. The organic fluorophores commonly employed in single-molecule studies of biological systems display fast photobleaching, intensity fluctuations on the millisecond timescale (blinking), or both. These phenomena limit observation time and complicate the interpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16–18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and
Pindex Conference proceedings 2009
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Ribosome: an Ancient Cellular Nano-Machine for Genetic Code Translation,ve ribosomes illuminated the significant of PTC mobility and supported the hypothesis that the ancient ribosome produced single peptides bonds and non-coded chains, utilizing nucleotide conjugated amino acids. Genetic control of the reaction evolved after polypeptides capable of enzymatic function w
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https://doi.org/10.1007/978-1-59745-240-3ture determination, mapping of intermolecular interactions, exploring fundamental biological mechanisms, following compound cycling in the environmental, analysis of synthetic solid compounds, and microimaging of a model organism.
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https://doi.org/10.1007/978-1-4614-7780-8f reducing potential. In contrast, anti-oxidants stabilize the Cy3 and Alexa488 fluorophores. We recommend use of the protocatechuate-3,4,-dioxygenase system with antioxidant additives, and in the absence of biological reducing agents. This system should have wide application to single-molecule fluo
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Cheryl P. Andam,Johann Peter Gogarten PhDve ribosomes illuminated the significant of PTC mobility and supported the hypothesis that the ancient ribosome produced single peptides bonds and non-coded chains, utilizing nucleotide conjugated amino acids. Genetic control of the reaction evolved after polypeptides capable of enzymatic function w
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Conference proceedings 2009rpretation of fl- rescence fluctuations [14, 15]. Molecular oxygen (O) modulates dye stability. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16–18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and
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發(fā)表于 2025-3-23 09:06:28 | 只看該作者
1874-6500 lity. Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. This results in the for- tion of singlet oxygen [16–18]. Singlet O reacts efficiently with organic dyes, 2 amino acids, and 978-90-481-2367-4978-90-481-2368-1Series ISSN 1874-6500 Series E-ISSN 1874-6535
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