Titlebook: Biophysical and Biochemical Aspects of Fluorescence Spectroscopy; T. Gregory Dewey Book 1991 Springer Science+Business Media New York 1991

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https://doi.org/10.1007/978-3-030-20511-9e the fundamental considerations and to provide the reader with a feeling for the practical aspects that must be apprehended before time-resolved methods can be profitably applied to complex biological systems.

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lly occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex- ploited with great success utilizing both intrinsic and extrinsic probes. Re- cent applications have built upon these traditional st

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Luminescent Trivalent Lanthanides in Studies of Cation Binding Sites, radius and, as such, are useful as steric probes of systems such as ion channels. Second, their paramagnetic and luminescent properties make them versatile spectroscopic probes of cation binding sites.

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Time-Resolved Fluorescence in Biology and Biochemistry,e the fundamental considerations and to provide the reader with a feeling for the practical aspects that must be apprehended before time-resolved methods can be profitably applied to complex biological systems.

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Fluorescence Quenching Reactions,ormational changes in the biomacromolecule can then be monitored in terms of changes in the accessibility to the quencher of the fluorophore. In addition to such topographical information, in some cases information about the conformational dynamics of globular proteins has been obtained with solute quenching reactions.