Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre

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Aufbau der Arbeit und Vorgehensweisead in mice. Both subcutaneous and orthotopic tumor models are readily observed with high sensitivity and reproducibility. User-friendly commercial instruments exist and, increasingly, luciferase-expressing tumor cells are available in academic institutions or commercially. There is an increasing lit

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Komplexit?t interner Dienstleistungenon-target tissues. In this study, a “smart” activatable strategy for specific tumor imaging is proposed in which pH-activatable targeted probes specifically detect tumors after binding to the target cell surface proteins, internalization, and eventual acidic pH activation within the acidic organelle

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https://doi.org/10.1007/978-3-658-11512-8mitting quantum dots provide excellent definition of vasculature, lymphoid organs, and lymph nodes draining both normal tissues and tumors. We detail methods for use with commercially available quantum dots and discuss common difficulties.

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https://doi.org/10.1007/978-3-642-60841-4se, including monitoring the fate of transplanted islets in vivo in real time. In order to advance our understanding of the pathophysiology and immunobiology of islet transplantation as they occur in living animals, islet grafts tagged with light-emitting luciferase can be implanted in a mouse islet

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https://doi.org/10.1007/978-3-642-60841-4imals to determine viral or bacterial distributions and titers. The recent application of the in vivo bioluminescence imaging (BLI) to monitor the replication and tropism of pathogens expressing the luciferase (from firefly or .) reporter proteins has been recently developed. This technology do not

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Kernf?higkeit: Wissensmanagements of viral infection in mice rely upon euthanizing cohorts of animals at multiple time points to identify sites of infection, quantify viral titers, and determine host immune responses. This experimental paradigm precludes longitudinal studies of infection and response to treatment in the same anima

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Günther Schuh,Hans-Peter Wiendahlrendered bioluminescent, allowing the sensitive detection of bacterial burden and metabolic activity in real-time and in situ in living animals. In this chapter we describe the protocols for characterizing in vivo infection models using bioluminescent bacteria: from real-time imaging in living anima