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Titlebook: Biological Small Angle Scattering: Techniques, Strategies and Tips; Barnali Chaudhuri,Inés G. Mu?oz,Volker S. Urban Book 2017 Springer Nat

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期刊全稱Biological Small Angle Scattering: Techniques, Strategies and Tips
影響因子2023Barnali Chaudhuri,Inés G. Mu?oz,Volker S. Urban
視頻videohttp://file.papertrans.cn/188/187467/187467.mp4
發(fā)行地址Includes the principles and theoretical background of various SAS techniques.States practical aspects that range from sample preparation to data publication.Covers techniques for improving data qualit
學科分類Advances in Experimental Medicine and Biology
圖書封面Titlebook: Biological Small Angle Scattering: Techniques, Strategies and Tips;  Barnali Chaudhuri,Inés G. Mu?oz,Volker S. Urban Book 2017 Springer Nat
影響因子This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatograph
Pindex Book 2017
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How to Analyze and Present SAS Data for Publication,gical, functional and even time resolved conditions. However, reconstructing three dimensional structures from SAS data is inherently ambiguous, as no information about orientation and phase is available. In addition experimental artifacts such as radiation damage, concentration effects and incorrec
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SAS-Based Structural Modelling and Model Validation,ing inhomogeneities at low resolution. An important example of such systems are solutions of biological macromolecules. Rapid development in the methodology for solution scattering data interpretation and model building during the last two decades brought the analysis far beyond the determination of
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What Can We Learn from Wide-Angle Solution Scattering?,e (SAXS) data. It is possible to accurately predict WAXS scattering on the basis of atomic coordinate sets and thus use it as a means of testing molecular models constructed on the basis of crystallography, molecular dynamics (MD), cryo-electron microscopy or . modeling. WAXS data may provide insigh
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High Resolution Distance Distributions Determined by X-Ray and Neutron Scattering,heir chemistry, biological macromolecules undergo structural changes over distances ranging from atomic to micrometer scales. X-ray and neutron scattering provide three key assets for tackling this challenge. First, they may be conducted on solutions where the macromolecules are free to sample the c
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