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Titlebook: Biological Low-Voltage Scanning Electron Microscopy; Heide Schatten,James B. Pawley Book 2008 Springer-Verlag New York 2008 biology.cell.c

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期刊全稱Biological Low-Voltage Scanning Electron Microscopy
影響因子2023Heide Schatten,James B. Pawley
視頻videohttp://file.papertrans.cn/188/187379/187379.mp4
圖書封面Titlebook: Biological Low-Voltage Scanning Electron Microscopy;  Heide Schatten,James B. Pawley Book 2008 Springer-Verlag New York 2008 biology.cell.c
影響因子.Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM...Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images..
Pindex Book 2008
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https://doi.org/10.1007/978-3-8351-9105-1eam energy of 5 keV or lower, offers significant improvements in the lateral and depth spatial resolution compared to conventional beam energy operation of 10 keV and higher. This improvement must be offset against the limitations that the low-beam energy imposes upon analytical x-ray spectrometry.
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High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications chapter are 1) Visualization of sub-membranous cytoskeletal features using cytoskeleton stabilization and membrane extraction protocols. Two examples of different specimens in this section are (1.1) subpellicular cytoskeletal structures of the apicomplexan parasite ., and (1.2) submembraneous actin
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