Titlebook: Biological Aging; Methods and Protocol Trygve O. Tollefsbol Book 2007 Humana Press 2007 DNA.Evolution.Microarray.Mutation.Proteomics.Telome

Fulsome

Caloric Restriction and Life Span Determination of Yeast Cells, methods used to determine the replicative life span of single yeast “mother” cells and measure recombination frequency at the rDNA locus, and how to isolate and analyze the circular forms of DNA known as extrachromosomal rDNA circles (ERCs), which are a major cause of aging in . (.–.).

JUST

Identifying Genes that Extend Life Span Using a High-Throughput Screening System,steps in our bud-scar-sorting system are the use of small microbeads, which allows successive rounds of purification and regrowth of the mother cells (M-cell), and utilization of flow cytometry to sort and isolate cells with a longer lifespan based on the number of bud scars specifically labeled with WGA.

Magisterial

Application of DNA Microarray Technology to Gerontological Studies,icroarray platforms, with radioactive and nonradioactive (fluorescent) labeling, are detailed in the manuscript. These in-depth protocols provide a background for the technical aspects of everyday work with DNA microarrays.

反復(fù)無常

,Anspruchsberechtigte (§ 62 EStG),different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations.

極小

水槽

,Andere Leistungen für Kinder (§ 65 EStG),tein expression because studies illustrate that it is possible for the enzymes to undergo posttranslational physical changes in response to stimulation even though gene transcription remains unchanged. Here, we discuss an in vitro method to study protein expression of DNMTs in aging systems.

減至最低

Methods for Cell Sorting of Young and Senescent Cells,different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations.

小爭(zhēng)吵

Telomere Length Analysis,on methods have improved greatly in accuracy and sensitivity since the development of the conventional telomeric Southern blot. Here, we describe the different methodologies recently developed for telomere length quantification, as well as their potential applications for human aging studies.

吝嗇性

A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro,tein expression because studies illustrate that it is possible for the enzymes to undergo posttranslational physical changes in response to stimulation even though gene transcription remains unchanged. Here, we discuss an in vitro method to study protein expression of DNMTs in aging systems.

仔細(xì)閱讀

https://doi.org/10.1007/978-1-59745-361-5DNA; Evolution; Microarray; Mutation; Proteomics; Telomere; Termination; age; gene transcription; genes; hybri