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Titlebook: Biolistic DNA Delivery in Plants; Methods and Protocol Sachin Rustgi,Hong Luo Book 2020 Springer Science+Business Media, LLC, part of Sprin

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樓主: purulent
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發(fā)表于 2025-3-30 10:23:45 | 只看該作者
Choice of Explant for Plant Genetic Transformationnot dependent on the cell type, species, and genotype. The success of any transformation technique depends on the starting experimental materials or the explants. Here, we describe the factors that have influenced the choice of explants in biolistic transformation. Many general factors in the select
52#
發(fā)表于 2025-3-30 15:41:32 | 只看該作者
53#
發(fā)表于 2025-3-30 17:37:36 | 只看該作者
Nanobiolistics: An Emerging Genetic Transformation Approache relatively large micron-scale biolistic projectiles can result in tissue damage, low regeneration efficiency, and create difficulties for the biolistic transformation of isomorphic small cells or subcellular target organelles (i.e., mitochondria and plastids). As an alternative to micron-sized car
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發(fā)表于 2025-3-31 00:45:20 | 只看該作者
Biolistic Transformation of Japonica Rice Varieties. One of the methodologies for genetic improvement is biolistic delivery of genetic components into plant cells. In this chapter, we describe steps involved in introducing plasmid DNA carrying gene of interest into rice mature embryos using Biolistic. PDS-1000/He particle delivery system. We also pr
55#
發(fā)表于 2025-3-31 03:53:31 | 只看該作者
56#
發(fā)表于 2025-3-31 05:31:31 | 只看該作者
Biolistic DNA Delivery and Its Applications in y, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since th
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