| 期刊全稱 | Biolistic DNA Delivery | | 期刊簡稱 | Methods and Protocol | | 影響因子2023 | Stephan Sudowe,Angelika B. Reske-Kunz | | 視頻video | http://file.papertrans.cn/188/187278/187278.mp4 | | 發(fā)行地址 | Covers biolistic gene transfer into plants, nematodes, or in vitro and in vivo mammalian cells.Includes lab-ready protocols with clear step-by-step direction from leading researchers in the field.Feat | | 學科分類 | Methods in Molecular Biology | | 圖書封面 |  | | 影響因子 | .Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun.? .Biolistic DNA Delivery: Methods and Protocols. provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful .Methods in Molecular Biology.? series format, chapters include introductions to their respective topics, lists of the necessary materials and | | Pindex | Book 2013 |
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