Titlebook: Biofuels from Algae; Methods and Protocol Kristian Spilling Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 fl

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Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Sampleser characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.

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Book 2020h, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and

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Complete Acid-Based Hydrolysis Assay for Carbohydrate Quantification in Seaweed: A Species-Specificl acid hydrolysis protocol for accurate quantification of the carbohydrate content of seaweeds. Different species of seaweed can be accurately evaluated for their carbohydrate contents by following this optimized method.

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Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterificatioix and then liberating the fatty acids to make these available for catalytic upgrading to fatty acid methyl esters (FAMEs), subsequent extraction into hexane, and then quantification by gas chromatography. The method we describe here requires less than 10?mg of biomass per sample and is considered high-throughput and highly accurate.

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,Kronprinzessin Luise von Preu?en,od (based on aldehyde functional group derivatization), for an overall combined monomeric sugar concentration determination, or a liquid chromatography method, for a more specific monomeric sugar profile.