Titlebook: Basic Protein and Peptide Protocols; John M. Walker Book 1994 Humana Press 1994

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Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dime the very high electroendosmotic flow caused by charged groups on the glass walls of the gel tubes. As a consequence, pH gradients are unstable and rarely extend above pH 8.0, leading to loss of basic proteins from 2-D maps. Gradients can be extended to pH 10 by special treatment of the glass IEF tubes (.) or by using horizontal flat-bed IEF (..).

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Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the Firstsing (IEF) gels containing 9. urea and 2% (w/v) of the detergent NP-40 with discontinuous polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE) in the second dimension. This technique is described in . and is reviewed in refs. .–..

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Financial Aspects of HCs Business Models,taining of proteins following gel electrophoresis was a major advance providing a detection sensitivity between 20–200 times higher than methods using CBB R-250, being able to detect about 0.1 ng protein/band.