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Titlebook: Basic DNA and RNA Protocols; Adrian J. Harwood Book 1996 Humana Press 1996

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樓主: GUST
31#
發(fā)表于 2025-3-26 23:14:26 | 只看該作者
Northern Blot Analysislar in vivo system being examined. This chapter details the method for analyzing RNA by Northern blotting, which basically involves the isolation of RNA, its size fractionation by electrophoresis, transfer to membrane, and detection by nucleic acid hybridization and autoradiography.
32#
發(fā)表于 2025-3-27 02:43:27 | 只看該作者
33#
發(fā)表于 2025-3-27 06:34:55 | 只看該作者
Techniques in Molecular Hematology, the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates. In this chapter, the preparation of the DNA templates and the transcription from the template of .P-labeled synthetic RNA molecules, commonly called riboprobes, will be discussed.
34#
發(fā)表于 2025-3-27 12:35:10 | 只看該作者
H. J. Deeg,D. T. Bowen,C. Niemeyer-stranded DNA probes were used for the assay (.). However, these have the disadvantage of being lengthy to prepare. The ease with which labeled RNA molelcules (riboprobes; ..) can now be made makes an assay based on RNA RNA hybridization much more favorable (.,.).
35#
發(fā)表于 2025-3-27 17:39:26 | 只看該作者
36#
發(fā)表于 2025-3-27 18:33:19 | 只看該作者
The Preparation of Riboprobes the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates. In this chapter, the preparation of the DNA templates and the transcription from the template of .P-labeled synthetic RNA molecules, commonly called riboprobes, will be discussed.
37#
發(fā)表于 2025-3-28 01:48:54 | 只看該作者
The RNase Protection Assay-stranded DNA probes were used for the assay (.). However, these have the disadvantage of being lengthy to prepare. The ease with which labeled RNA molelcules (riboprobes; ..) can now be made makes an assay based on RNA RNA hybridization much more favorable (.,.).
38#
發(fā)表于 2025-3-28 02:30:19 | 只看該作者
https://doi.org/10.1385/1592590748er sensitivity applications, and the combination of fluorescein-labeling and enhanced chemiluminescent detection allows as little as 20 × 10. mol of homologous target to be detected within a 1-h exposure.
39#
發(fā)表于 2025-3-28 09:40:27 | 只看該作者
40#
發(fā)表于 2025-3-28 14:01:28 | 只看該作者
Hematologic Cytology of Storage Diseasesatured by sequential soaking of the gel in solutions containing HCl and NaOH, respectively. The denatured DNA fragments are then transferred to a solid matrix or filter (usually a nylon membrane) for subsequent hybridization to a specific labeled probe (.).
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