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Titlebook: Basic Cloning Procedures; Valdis Berzins Book 1998 Springer-Verlag Berlin Heidelberg 1998 DNA.RNA.gene.nucleic acid.protein.protein synthe

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期刊全稱Basic Cloning Procedures
影響因子2023Valdis Berzins
視頻videohttp://file.papertrans.cn/181/180970/180970.mp4
發(fā)行地址Basic laboratory techniques, approved in several international molecular biology courses.Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information.Th
學(xué)科分類Springer Lab Manuals
圖書封面Titlebook: Basic Cloning Procedures;  Valdis Berzins Book 1998 Springer-Verlag Berlin Heidelberg 1998 DNA.RNA.gene.nucleic acid.protein.protein synthe
影響因子This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
Pindex Book 1998
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?An dem Webstuhl des Gedankens“ gel electrophoresis and then transferred from the gel to a filter so that their relative positions are preserved. The fragments of interest are identified by hybridization with a complementary nucleic acid probe.
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https://doi.org/10.1007/978-3-662-64170-5ion mix and used for subsequent studies. Commercially available in vitro translation extracts also allow the co-translation of several different proteins simultaneously, thus providing a system to study protein:protein interactions as well as protein:DNA and protein: RNA interactions.
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Protein Synthesis in Cell-Free Systems,ion mix and used for subsequent studies. Commercially available in vitro translation extracts also allow the co-translation of several different proteins simultaneously, thus providing a system to study protein:protein interactions as well as protein:DNA and protein: RNA interactions.
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cDNA Synthesis and Cloning,alysis and in situ hybridization. Another important application of RNA templates is the construction of representa-tive cDNA libraries in order to clone genes, to investigate their molecular structure and to express them in prokaryotic and/or eukaryotic cells (Belyaysky 1989).
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