Titlebook: Basic Cell Culture Protocols; Cheryl D. Helgason,Cindy L. Miller Book 20053rd edition Humana Press 2005 DNA.Macrophages.cell.cell culture.

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Human and Mouse Hematopoietic Colony-Forming Cell Assays,progenitors that can be detected in vitro using colony-forming cell (CFC) assays. Hematopoietic progenitor cells, when cultured in a semisolid methylcellulose-based medium that is supplemented with suitable growth factors, proliferate and differentiate to produce clonal clusters (colonies) of maturi

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Hematopoietic Development of Human Embryonic Stem Cells in Culture,s of human blood development. This chapter describes two methods to promote hematopoietic differentiation of human ES cells: stromal cell coculture and embryoid body formation. Better understanding of basic human hematopoiesis through the study of human ES cells will likely have future therapeutic b

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Generation of Murine Stromal Cell Lines,rom subdissected AGM regions in “explant-” or “single-cell suspension”-type cultures from embryos transgenic for ., a temperature-sensitive mutant of the SV40 T antigen gene. The number of cells in such cultures initially expand, but in most cases, this expansion phase is followed by a stable or eve

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Culture of Human and Mouse Mesenchymal Cells,onment cells, colony-forming-unit fibroblasts (CFU-F), and mesenchymal cells. These cells were originally thought to provide an appropriate matrix for hematopoietic cell development, but recent examination of these cell populations suggests a much broader spectrum of activity, including the generati