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Titlebook: Genetic Transformation Systems in Fungi, Volume 1; Marco A. van den Berg,Karunakaran Maruthachalam Book 2015 Springer International Publis

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發(fā)表于 2025-3-21 19:32:42 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Genetic Transformation Systems in Fungi, Volume 1
編輯Marco A. van den Berg,Karunakaran Maruthachalam
視頻videohttp://file.papertrans.cn/383/382641/382641.mp4
概述The most comprehensive book on fungal transformation methods.The best protocols to be applied in your lab.Includes the latest tools and techniques
叢書(shū)名稱(chēng)Fungal Biology
圖書(shū)封面Titlebook: Genetic Transformation Systems in Fungi, Volume 1; Marco A. van den Berg,Karunakaran Maruthachalam Book 2015 Springer International Publis
描述.Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ?.Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (.Phytophthora. and .Botrytis.) and mammalian infection (.Penicillium marneffei. and .Candida.). .Genetic Transformation Systems in Fungi, Volume 1 .provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits. .
出版日期Book 2015
關(guān)鍵詞fungi; protocols; reviews; tools; transformation; Systems Biology
版次1
doihttps://doi.org/10.1007/978-3-319-10142-2
isbn_softcover978-3-319-37970-8
isbn_ebook978-3-319-10142-2Series ISSN 2198-7777 Series E-ISSN 2198-7785
issn_series 2198-7777
copyrightSpringer International Publishing Switzerland 2015
The information of publication is updating

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Defining the Maqasid for Measurementof efficient transformation systems has been hampered by the extreme diversity of ascomycetes, basidiomycetes, and zygomycetes. Polyethylene glycol-assisted transformation of fungal protoplasts is the most widely used system but alternative procedures, e.g., electroporation and .-mediated transforma
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Intestinal Fluid Handling: Secretion genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..
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Beyond Subordination: Four Arguments into electro-competent (E-comp) . cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (
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