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Titlebook: Base Editors; Methods and Protocol Sangsu Bae,Beomjong Song Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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樓主
發(fā)表于 2025-3-21 17:56:50 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Base Editors
期刊簡稱Methods and Protocol
影響因子2023Sangsu Bae,Beomjong Song
視頻videohttp://file.papertrans.cn/181/180909/180909.mp4
發(fā)行地址Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Base Editors; Methods and Protocol Sangsu Bae,Beomjong Song Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive lice
影響因子This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Base Editors: Methods and Protocols. serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields..
Pindex Book 2023
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沙發(fā)
發(fā)表于 2025-3-21 21:29:39 | 只看該作者
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Delivering Base Editors In Vivo by Adeno-Associated Virus Vectorstissues. We provide step by step protocols for (i) designing and validating base editing systems, (ii) packaging base editors into recombinant AAV vector particles, (iii) delivering AAV to the central nervous system via intrathecal injection, and (iv) quantifying base editing frequencies by next-generation sequencing.
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發(fā)表于 2025-3-22 08:46:30 | 只看該作者
Maximum Likelihood Hebbian Learningning-based computational models to predict the efficiencies and outcome frequencies of ABE and CBE at given target DNA sites, in silico. Here, we describe the step-by-step procedure for the accurate determination of specific target nucleotides for ABE or CBE editing on the online available web tool, (DeepBaseEditor, .).
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發(fā)表于 2025-3-23 02:52:10 | 只看該作者
Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seqving uracil in vitro. Digested genomic DNA is subjected to WGS, and then sequencing reads are aligned to the reference genome, resulting in straight alignments at on-target and off-target sites. The in vitro cleavage sites related to the straight alignments can be identified using the Digenome-seq computer tool.
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發(fā)表于 2025-3-23 08:08:29 | 只看該作者
Functional Analysis of Variants in BRCA1 Using CRISPR Base Editors introduced the target nucleotide substitution in living cells and identified variants whose functions were not defined. Here, we describe the methods for the functional appraisal of BRCA1 variants using CRISPR-based base editors.
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