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Titlebook: Baculovirus and Insect Cell Expression Protocols; David W. Murhammer Book 2016Latest edition Springer Science+Business Media, LLC 2016 BEV

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發(fā)表于 2025-3-21 20:06:36 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
期刊全稱Baculovirus and Insect Cell Expression Protocols
影響因子2023David W. Murhammer
視頻videohttp://file.papertrans.cn/181/180359/180359.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
學(xué)科分類Methods in Molecular Biology
圖書(shū)封面Titlebook: Baculovirus and Insect Cell Expression Protocols;  David W. Murhammer Book 2016Latest edition Springer Science+Business Media, LLC 2016 BEV
影響因子.The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Baculovirus and Insect Cell Expression Protocols, Third Edition. aims to not only aid the user in succ
Pindex Book 2016Latest edition
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發(fā)表于 2025-3-21 20:41:13 | 只看該作者
Annika Wilhelmy,Martin KleinmannThis chapter lists the known cell lines from Lepidoptera, largely based on previous compilations of insect cell lines published by W. Fred Hink. More than 320 lines from 65 species are listed. The official designation is given for each cell line as well as the species, tissue source, and, when known, the susceptibilities to baculoviruses.
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發(fā)表于 2025-3-22 02:34:58 | 只看該作者
Annika Wilhelmy,Martin KleinmannThis chapter describes procedures for initiating new cell lines from lepidopteran larval tissues and embryos. The internal morphology is described along with methods for treating excised tissues and the primary cultures. Advice on culture medium and the tissues that will provide the best chance for new cell lines is discussed.
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發(fā)表于 2025-3-22 06:26:55 | 只看該作者
Available Lepidopteran Insect Cell LinesThis chapter lists the known cell lines from Lepidoptera, largely based on previous compilations of insect cell lines published by W. Fred Hink. More than 320 lines from 65 species are listed. The official designation is given for each cell line as well as the species, tissue source, and, when known, the susceptibilities to baculoviruses.
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發(fā)表于 2025-3-22 20:38:24 | 只看該作者
Heinz Kindler,Adelheid Unterstallernsect cell system. The production and scale-up of these particles, which are mostly sought as vaccine candidates, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. The use of host insect cells allows mass production of VLPs in a proven scalable system.
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發(fā)表于 2025-3-23 00:33:06 | 只看該作者
Karl Poppers Sp?twerk und seine ?Welt 3“ model system (e.g., for studying apoptosis). The fundamental techniques involved in these applications are described throughout this book. In this chapter the most widely used techniques are summarized and the reader is directed to detailed information found elsewhere in this book. Furthermore, man
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發(fā)表于 2025-3-23 04:42:12 | 只看該作者
Karl Popper und Friedrich August von Hayektion in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure and their replication in insect larvae. Baculovirus gene expression occurs in an ordered cascade, regulated by early, late
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