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Titlebook: Baculovirus Expression Protocols; Christopher D. Richardson Book 19951st edition Humana Press 1995

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發(fā)表于 2025-3-21 16:33:54 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Baculovirus Expression Protocols
影響因子2023Christopher D. Richardson
視頻videohttp://file.papertrans.cn/181/180357/180357.mp4
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Baculovirus Expression Protocols;  Christopher D. Richardson Book 19951st edition Humana Press 1995
Pindex Book 19951st edition
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書目名稱Baculovirus Expression Protocols影響因子(影響力)




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Insect Cell Culture in Serum- Free Media,ntial replication of entomopathogenic viruses in vitro, there have been over 400 cell lines established to date from various insect species (.). Without exception, the cell lines described in the literature and currently in use have been isolated and established from primary cultures using serum-sup
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Transfection Techniques for Producing Recombinant Baculoviruses,es for foreign gene expression. A variety of transfection methods, which were first developed for mammalian cell lines, have been adapted for use with insect tissue-culture cells. Conditions required for the maximum transfection efficiency of any vertebrate or invertebrate cell line vary considerabl
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Selection of Recombinant Baculoviruses by Visual Screening,on of recombinants is rapid compared to mammalian expression systems, whereas the capacity to perform many posttranslational modifications necessary for the expression of authentic, functional proteins is retained. Examples of glycosylation (.), phosphorylation (.), myristilation (.), palmitoylation
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Production of recombinant baculoviruses using linearized viral DNA,enome with the corresponding segment from a transfer vector that has been modified to include a foreign gene. The frequency of this recombination is low; most of the input viral DNA molecules do not recombine with a plasmid DNA. Typically, only 0.1-2% of the progeny viruses are recombinant; hence, l
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