Titlebook: Bacteriophages; Methods and Protocol Martha R. J. Clokie,Andrew Kropinski,Rob Lavigne Book 2019 Springer Science+Business Media, LLC, part

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,Analyzing Phage–Host Protein–Protein Interactions Using ,-tag, II Purifications, purification of the tagged protein which allows the copurification of all bacterial and phage specific interacting proteins. After SDS-PAGE analysis and an in-gel trypsin digestion, the purified interacting proteins are identified by mass spectrometry analysis. The identification of phage proteins

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Screening for Growth-Inhibitory ORFans in ,-Infecting Bacteriophages,-inducible promoter. Furthermore, we describe a method to examine the effect of the phage proteins in different hosts, using different vector copy numbers. Finally, we explain how to investigate the effect of ORFan expression on the host morphology using time-lapse microscopy.

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Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria,of revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of . using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.

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Engineering Bacteriophage-Based Biosensors, a narrow subset of diagnostic settings. More recently, however, advances in DNA sequencing and the introduction of more sensitive reporter systems have enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to enginee

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Site-Specific Mutagenesis of , Phage SPO1,rgeted gene is cloned in a . shuttle vector. Using an in vitro enzymatic procedure dependent on mutant oligonucleotide primers, a mutation is inserted into the cloned gene, replacing an early lysine codon (AAA or AAG) with a nonsense codon (TAG or TAA). The mutant plasmid is recovered by transformat