Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2024Latest edition The Editor(s) (if applicable) an

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1064-3745 ation advice from the experts.This second edition details new and updated methods used for studying prokaryotic non-coding RNAs and their protein accomplices.?Chapters detail discovery of ncRNAs, characterization of their structure, functions, and their interactomes.?Written in the highly successful

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Handbuch Datenschutz und IT-Sicherheitolate vesicles in order to understand their biological functions. Here we describe a polymer-based precipitation method allowing high-yield isolation of extracellular vesicles and their cargo RNA from the Gram-positive bacterium ..

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Handbuch Demokratische Republik Kongo previous studies, the sRNA predictions are accurate and reliable. In this chapter, we outline the sRNA detection algorithm, important parameters used, step-by-step execution, and data interpretation with a . study as an example. Following those procedures, novel sRNA can be revealed by ANNOgesic.

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Experimental Validation of RNA–RNA Interactions by Electrophoretic Mobility Shift Assaycomplex formation. Next, base pairing is restored by introducing complementary mutations in its partner RNA. Here, we describe the mutational strategy and experimental methods used to validate specific base pairing between two RNA species.

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Directed Screening for sRNA Targets in , Using a Plasmid LibraryA signaling is identifying the sRNA regulators of specific genes. Here, we describe a protocol for making an sRNA expression library and using this library to screen for sRNA regulators of genes of interest in .. This library can be easily expanded and adapted to use in other bacteria.