Titlebook: Bacterial Protein Secretion Systems; Methods and Protocol Laure Journet,Eric Cascales Book 2017 Springer Science+Business Media LLC 2017 Fr

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1064-3745 ation advice from the experts.This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifyin

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Birger P. Priddat,Christoph Meinekeryl-prolipoprotein that in turn converts into apolipoprotein, which is finally converted into mature triacylated lipoprotein. Here we describe the detection of one of these intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using .H-palmitate labeling and inhibition by globomycin and detection by fluorography.

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https://doi.org/10.1007/978-3-658-26431-4 of the peptidoglycan. Their activities are spatially controlled to avoid cell lysis and to create localized rearrangement of the cell wall. This is assured by interaction with the structural subunits of the apparatus. Here we describe protocols to test the peptidoglycan hydrolase activity of these proteins in vitro and in solution.

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Defining Membrane Protein Localization by Isopycnic Density Gradients, for the purification of outer and inner membranes from .. The procedure can be applied with minor modifications to other bacterial species, including those carrying capsular polysaccharide attached to the outer membrane.

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