Titlebook: Bacterial Polysaccharides; Methods and Protocol Inka Brockhausen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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Intact

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Focus-Words

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GRAIN

https://doi.org/10.1007/978-3-531-92639-1eptors followed by detection of .-nitrophenolate. GT reactions coupled with phosphatases and detection of inorganic phosphate are suitable for most GTs. These assays permit convenient quantification of GT activities and kinetics without the use of radioactive sugars.

SOB

Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria,ical characterization. This protocol describes the hydrofluoric acid extraction and purification of the secondary cell wall polymer of . that is also found in the envelope of the other members of the . sensu lato group of bacteria.

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Glycosyltransferase Activity Assay Using Colorimetric Methods,eptors followed by detection of .-nitrophenolate. GT reactions coupled with phosphatases and detection of inorganic phosphate are suitable for most GTs. These assays permit convenient quantification of GT activities and kinetics without the use of radioactive sugars.

SMART

Book 2019ures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria,

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