Titlebook: Bacterial Persistence; Methods and Protocol Jan Michiels,Maarten Fauvart Book 2016 Springer Science+Business Media New York 2016 Antibiotic

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Functional Analysis of the Role of Toxin–Antitoxin (TA) Loci in Bacterial Persistencebinant TA genes into inducible plasmids and expressing these in . Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in . and whether expression can affect persister cell formation.

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Experimental Evolution of Escherichia coli Persister Levels Using Cyclic Antibiotic Treatmentsattempts have been made to elucidate persistence mechanisms. However, because of the challenges involved in studying persisters and the clear redundancy in mechanisms underlying their generation, our knowledge of molecular pathways to persistence remains incomplete. Here, we describe how to use expe

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In Vitro Models for the Study of the Intracellular Activity of Antibioticspotent or effective against intracellular bacteria as well as to explore the reasons for poor bacterial responsiveness. An in vitro pharmacodynamic model is described, consisting of (1) phagocytosis of pre-opsonized bacteria by eukaryotic cells; (2) elimination of non-internalized bacteria with gent

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https://doi.org/10.1007/978-1-4939-2854-5Antibiotics; Bacteria; Mycobacterium Tuberculosis; Persister Cells; Pseudomonas Aeruginosa; Staphylococcu

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978-1-4939-4904-5Springer Science+Business Media New York 2016

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Persisters: Methods for Isolation and Identifying Contributing Factors—A ReviewPersister cells are phenotypic variants surviving a lethal dose of antibiotic, sufficient to kill the bulk of an exponential phase population. In this chapter we summarize current techniques to isolate persisters and discuss limitations associated with identifying mechanisms of persister formation.

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