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Titlebook: Bacterial Artificial Chromosomes; Kumaran Narayanan Book 2015Latest edition Springer Science+Business Media New York 2015 BAC construction

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發(fā)表于 2025-3-21 19:27:16 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
期刊全稱Bacterial Artificial Chromosomes
影響因子2023Kumaran Narayanan
視頻videohttp://file.papertrans.cn/181/180255/180255.mp4
發(fā)行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
學(xué)科分類Methods in Molecular Biology
圖書封面Titlebook: Bacterial Artificial Chromosomes;  Kumaran Narayanan Book 2015Latest edition Springer Science+Business Media New York 2015 BAC construction
影響因子.Bacterial Artificial Chromosomes, Second Edition. expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls..Authoritative and cutting-edge, .Bacterial Artificial Chromosomes, Second Edition .seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies..
Pindex Book 2015Latest edition
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沙發(fā)
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Retinal Vascular Characteristicslleles by conventional methods like Southern Blotting. Recently, we introduced a novel BAC targeting strategy, in which Restriction Fragment Length Polymorphisms (RFLPs) are targeted in polymorphic mouse ES cells, enabling an efficient and easy PCR-based readout to identify properly targeted alleles
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MacIntyre’s Influence on Business Ethicsnome. (2) Introduction of the transfer plasmid sequences into the alphaherpesvirus genome via homologous recombination in mammalian cells. (3) Isolation of recombinant virus genomes containing the BAC backbone sequences from infected mammalian cells and electroporation into .. (4) Preparation of inf
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https://doi.org/10.1007/978-94-007-6729-4 be applied on large inborn or acquired derivative chromosomes. The main feature of this set is that the probes are applied in a chromosome-specific manner and they align along the chromosome in average intervals of ten megabasepairs. Hence PCL-FISH provides denser coverage and a more precise anchor
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發(fā)表于 2025-3-22 21:21:45 | 只看該作者
Recombineering Linear BACsere structures. Linear BACs may be useful for functional expression of genomic loci in cells, maintenance of linear viral genomes in their natural conformation, and for constructing innovative artificial chromosome structures for applications in mammalian and plant cells.
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發(fā)表于 2025-3-23 02:30:22 | 只看該作者
Making BAC Transgene Constructs with Lambda-Red Recombineering System for Transgenic Animals or Cellnotyping, BAC coverage and integrity as well as quality of BAC DNA will be addressed. Not only does this approach provide a practical and effective way to modify large DNA constructs, the same recombineering principles can apply to smaller high copy plasmids as well as to chromosome engineering.
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發(fā)表于 2025-3-23 07:36:47 | 只看該作者
Directing Enhancer-Traps and iTol2 End-Sequences to Deleted BAC Ends with loxP- and lox511-Tn10 Tranis procedure are suitable for numerous mapping studies. Because DNA in front of the loxP (or lox511) arrowheads in the Tn10 transposon is retained at the newly created BAC end, exogenous DNA cassettes such as enhancer-traps and iTol2 ends can be efficiently introduced into BAC ends for germline expr
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