Titlebook: B Cell Protocols; Hua Gu,Klaus Rajewsky Book 2004 Humana Press 2004

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https://doi.org/10.1007/978-3-319-76526-6which developmentally occur at different cellular stages. Various in vitro models have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreo

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Hua Gu,Klaus RajewskyIncludes supplementary material:

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Synthetic Fingerprint Generation,s A–F) and the functional B-cell subsets (B-1a, B-1b, B-2, and marginal zone [MZ] B cells) in the periphery..Although we focus on murine B-cell subsets, the methods we discuss are relevant to FACS studies conducted with all types of cells and other FACS instruments. We introduce a new method for sca

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Brian J. Meacham,Margaret McNameetransfer into recipient mice. The localization of transferred cells in lymphoid organs can be determined by immunohistochemistry and/or flow cytometric analysis of harvested tissues or cells. Because the proper localization of B cells in lymphoid organs is a multistep process, abnormal positioning c

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M. L. Parsons,B. W. Smith,G. E. Bentleyfrom single cells within the population to estimate the level of somatic hypermutation as the best molecular indicator of B-cell memory (.). In this chapter, we describe the methods used in each of these four sections that serve to provide high-resolution quantification of antigen-specific B-cell me

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In Vitro and In Vivo Assays of B-Lymphocyte Migration,transfer into recipient mice. The localization of transferred cells in lymphoid organs can be determined by immunohistochemistry and/or flow cytometric analysis of harvested tissues or cells. Because the proper localization of B cells in lymphoid organs is a multistep process, abnormal positioning c